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The role of B-cell specific transcription factors in Hodgkin lymphoma
PAX5 is a transcription factor, expressed during most stages of B-cell differentiation and down-regulated during plasma cell development. In turn, the transcription factor IRF4 is being up-regulated during plasma cell differentiation. The co-expression of both transcription factors is not found in normal B- or plasma cells but is observed in more than 90% of classical Hodgkin lymphomas (cHL) and approximately 50% of diffuse large B-cell lymphomas (DLBCL), although both lymphomas show a completely different phenotype.
We examine the genome-wide binding profile and function of PAX5 and IRF4 in cHL and DLBCL cell lines. Our aim is not only to determine new target genes of both transcription factors, but also to resolve why PAX5 is not able to restore the B-cell phenotype in cHL and which role IRF4 plays during plasma cell differentiation.
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